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1. To determine whether the constructed plasmid was efficientworks well, we first transfected the plasmid with liposomes into cultured SH-SY5Y cells for 24 hours and analyzed the expression of corresponding proteins byused western blot analysis using anwith antibody against EGFP to check the expression of corresponding proteins.

2. We screened aA pool of Arabidospis Arabidopsis lines transformed with the activation vector was screened for short hypocotyl mutants under dim far-red light. Consequently we isolated, and three mutant lines designated chibi1-3 (chi) were isolated.

3. Rats were sacrificed killed at different time points followingof reperfusion. Left hemispheric Ffrontal and parietal lobe cortex tissues on the left hemisphere (6 to 11 mm fromto the tip of olfactory bulb) were separated and saved in liqguid nitrogen.

4. Rats which were supplemented with further injections as necessary during experiments to sustain a level of acceptable anesthesia. Body temperature was maintained at 36-37°C using a heating pad placed underneath the rat. . Each rat’sThe heads was wasere fixed horizontally in a stereotaxic instrument (Narishige, Japan). The skull was exposed, and a small burr hole was made at the appropriate coordinates drilled to enable vertical penetration by the stimulating and recording electrodes. All electrodes were zeroed on the at bregma, and all coordinates were calculated from this point.

5. We These experiments demonstrated a distinctive localization of OMPFC neurons, which revealed excitatory responses to BLA stimulation, although Pérez-Jaranay and Vivesa previous study did not demonstrate this apparent distribution of the excitatory responses (Pérez-Jaranay and Vives, 1991).

6. WHere we suggest that a comprehensive molecular genetic study involving a large number of NF2 is encouraged to build up our Chinese gene mutation database not only for the benefit for ofthe clinical practice, but also for genetic counseling purposess.

7. It is high reasonable to believe suggest that these NATs are critical for the life of humans, mouse mice and rats, just because they are stillremain in the transcriptome after tens of million years of evolution.

8. A total of 570 NATs were selected, tripled ofthree times the number lehner reported earlier in a previous study (8), and with most of them are thebeing reported for the first time to be reported.

9. It was noted that the onset of SWS1 expression in our study was earlier than the that one reported previously [26] where the expression initiateds between 55 and 60 hpf and with about half of the embryos showing expression at 60 hpf but are stillremaining in stage 1.  In theirThe aforementioned study differed from ours in that only fish that had achieved certain developmental characteristics at certain time points were examined for opsin expression to minimize the individual variation [26], while we did not perform any such selection.  The time difference discrepancy between the two studies is most likely to be due to this difference originate from it..

10. By constructing DB2GO, the entries in the BioDW member databases of BioDW are linked organically to the terms of Gene Ontology Database. organically. In the DB2GO table, Iit is quite easy to find entries in the DB2GO table from different databases as they, which all are all annotated by the same GO term, from different databases.

11. We screened aA pool of Arabidospis Arabidopsis lines transformed with the activation vector was screened for short hypocotyl mutants under dim far-red light. Consequently we isolated, and three mutant lines designated chibi1-3 (chi) were isolated.

12. [Author: You should mention the 3 treatment groups here since this is really part of the study design (like you did in the ‘Methods’ section but in less detail).]
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